Interfering with the Expression of Ubiquitin-Like with PHD and Ring Finger Domains 1 Can Inhibit the Invasion of Human Renal Cell Carcinoma

Objective. The ubiquitin-like with PHD and ring finger domains 1 (UHRF1) is a protein coding gene which associated colorectal cancer other diseases. Therefore, the present study was aimed at investigating effect mechanism of UHRF1 on invasion metastasis in human renal carcinoma cells. Methods. After interfered or overexpressed cell lines A498 769-P, relative mRNA level detected by RT-qPCR immunofluorescence. colony formation assay MTT were performed to observe proliferation viability each group. In addition, cells group Transwell wound healing assay. Finally, Western blot utilized measure expression MMP-2 MMP-9 Wnt/β-catenin signaling pathway. Results. ability, proliferation, invasion, 769-P inhibited after interfering UHRF1. MMP-2, MMP-9, c-myc, β-catenin significantly decreased, while GSK-3β increased. However, contrasting results demonstrated when overexpressed. Conclusions. Interference able inhibit may be related mediating pathway regulating MMP-9.